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BlueGene Biotech Canine Nerve Growth Factor ELISA kit (E08N0014)

E08N0014 Canine Nerve Growth Factor ELISA kit

The Canine Nerve Growth Factor ELISA kit can be used to identify samples from the Canine species. Nerve Growth Factor can also be called NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor, NGF.

Products

Specifications of Canine Nerve Growth Factor ELISA kit

Product Information

Cat. No.

E08N0014

Product Name

Canine Nerve Growth Factor ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NGF ELISA kit uses an anti-NGF antibody and an NGF-HRP conjugate in a competitive enzyme immunoassay method. NGF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NGF antibody binding site between NGF from samples and NGF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NGF. Since the number of sites is limited, as more sites are occupied by NGF from the sample, fewer sites are left to bind NGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NGF concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Nerve Growth Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NGF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Canine Nerve Growth Factor ELISA kit

Summary of the Assay Procedure for Canine Nerve Growth Factor ELISA kit

Citations of Canine Nerve Growth Factor ELISA kit

E08N0014 has been referenced in the below publications:

Effects of Electroacupuncture on Plasma Concentrations of Three Related Neurotrophic Factors in Normal Dogs.

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